In vitro embryo production in sheep: Pregnancy after long periods of oocyte and embryo transport

In vitro bovine embryo production in a synthetic medium: embryo development, cryosurvival, and establishment of pregnancy.

The aim of this study was to develop an in vitro embryo culture medium without either fetal calf serum or BSA, using various growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF-β1, and PDGF-BB), and other molecules with surfactant and embryotrophic properties, such as recombinant albumin (RA) and hyaluronan (HA). The first part of the study was dedicated to define the ...

Interstitial Pregnancy after In Vitro Fertilization and Embryo Transfer Following Bilateral Salpingectomy:Report of Two Cases and Literature Review

Heterotopic pregnancy after in vitro fertilization and embryo transfer.

We have presented a second case of heterotopic pregnancy after IVF-ET. The most likely cause is direct extrusion of embryos through the tubal ostia by the hydrostatic pressure associated with ET. The diagnosis of ectopic pregnancy must be suspected clinically and not ruled out on the sonographic demonstration of an intrauterine pregnancy. Early diagnosis is essential for the prevention of signi...

The effects of melatonin on in vitro oocyte competence and embryo development in sheep

The aim of this study was to assess the effects of melatonin on the in vitro maturation and fertilization of sheep oocytes, and on the in vitro culture of the embryos. Oocytes from sheep ovaries collected at the slaughterhouse were divided into four groups, two of which were treated with either 10 M (M5) or 10 M (M6) melatonin, while the other two groups served as untreated controls (C5 and C6)...

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...